LOCUS       MAN2B2-pGEc2            8253 bp    DNA     circular     21-SEP-2016
FEATURES             Location/Qualifiers
     misc_feature    complement(7731..7860)
                     /note="3'-ITR"
     misc_feature    complement(7429..7643)
                     /note="BGH pA"
     rep_origin      1137..1804
                     /dnas_title="Col E1 origin"
                     /label=Col E1 origin
                     /vntifkey="33"
     CDS             129..989
                     /dnas_title="AmpR"
                     /label=AmpR
                     /vntifkey="4"
     misc_feature    1998..2127
                     /note="5'ITR"
     misc_feature    2208..2790
                     /note="CMV Promoter"
     misc_feature    2828..2960
                     /note="Artificial Intron"
     misc_feature    6881..7422
                     /note="WPRE"
     misc_recomb     complement(2974..3001)
                     /label=attB1
                     /note="attB1 site"
                     /note="recognized as attB1"
     misc_feature    6060..6082
                     /note="attB2"
     misc_feature    6860..6865
                     /note="BstB1 site"
     misc_feature    6083..6778
                     /note="Super GFP"
     misc_feature    6779..6829
                     /note="AviTag"
     misc_feature    6830..6853
                     /note="His Tag"
     PCR_frame       3011..6059
                     /note="Reading Frame"
                     /set="Set 1"
     misc_feature    3011..6037
                     /note="OPTIMIZED MAN2B2 coding region"
     misc_feature    6038..6059
                     /note="TEV"
     vector          join(6068..8253,1..2991)
                     /source="pGEc2-DEST"
                     /type="Destination Vector"
     clone           2992..6067
                     /source="MAN2B2 pDONR"
                     /type="Entry Clone"
     source          1..8253
                     /dnas_title="MAN2B2-pGEc2"
ORIGIN      
        1 GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT
       61 CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA
      121 GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT
      181 GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT
      241 TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT
      301 TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG
      361 TATTATCCCG TATTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA
      421 ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA
      481 GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA
      541 CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA
      601 CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA
      661 CCACGATGCC TGTAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA
      721 CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC
      781 TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC
      841 GTGGTTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG
      901 TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA
      961 TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT
     1021 AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA
     1081 ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG
     1141 AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA
     1201 CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT
     1261 TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTTCTT CTAGTGTAGC
     1321 CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA
     1381 TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA
     1441 GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC
     1501 CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA
     1561 GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA
     1621 CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG
     1681 GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC
     1741 TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG
     1801 CTCATTAGGC ACCCCAGGCT TTACCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA
     1861 AGCGGAGAGC GCCCAATACG CAAGGAAACA GCTATGACCA TGTTAATGCA GCTGGCACGA
     1921 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGGAAGGCC CATGAGGCCA GTTAATTAAG
     1981 AGGTACCTTA ATTAAGGCTG CGCGCTCGCT CGCTCACTGA GGCCGCCCGG GCAAAGCCCG
     2041 GGCGTCGGGC GACCTTTGGT CGCCCGGCCT CAGTGAGCGA GCGAGCGCGC AGAGAGGGAG
     2101 TGGCCAACTC CATCACTAGG GGTTCCTTGT AGTTAATGAT TAACCCGCCA TGCTACTTAT
     2161 CTACGTAGCC ATGCTCTAGG AAGATCGCAA TTCGCCCTTA AGCTAGCTAG TTATTAATAG
     2221 TAATCAATTA CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT
     2281 ACGGTAAATG GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG
     2341 ACGTATGTTC CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT
     2401 TTACGGTAAA CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT
     2461 ATTGACGTCA ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG
     2521 GACTTTCCTA CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG
     2581 TTTTGGCAGT ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC
     2641 CACCCCATTG ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA
     2701 TGTCGTAACA ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC
     2761 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCTGC AGAAGTTGGT CGTGAGGCAC
     2821 TGGGCAGGTA AGTATCAAGG TTACAAGACA GGTTTAAGGA GACCAATAGA AACTGGGCTT
     2881 GTCGAGACAG AGAAGACTCT TGCGTTTCTG ATAGGCACCT ATTGGTCTTA CTGACATCCA
     2941 CTTTGCCTTT CTCTCCACAG GTGTCCAGCG GCCATCACAA GTTTGTACAA AAAAGCAGGC
     3001 TCGCGCCACC ATGGGCCAGC TGTGTTGGCT GCCTCTGCTG GCTCCACTGC TGCTGCTCAG
     3061 ACCTCCTGGT GTCCAGTCTG CCGGACCTAT CAGAGCCTTT GTGGTGCCCC ACTCTCACAT
     3121 GGATGTGGGC TGGGTGTACA CAGTGCAGGA ATCCATGAGA GCCTACGCCG CCAACGTGTA
     3181 CACTAGCGTG GTGGAAGAAC TGGCCAGAGG ACAGCAGCGG AGATTCATTG CCGTGGAACA
     3241 GGAATTCTTC CGGCTCTGGT GGGATGGCGT GGCCAGCGAT CAGCAGAAAT ATCAGGTGCG
     3301 ACAGCTGCTG GAAGAGGGCC GGCTGGAATT TGTGATCGGA GGCCAGGTCA TGCACGATGA
     3361 GGCCGTGACA CACCTGGACG ATCAGATCCT GCAGCTGACA GAGGGCCACG GCTTTCTGTA
     3421 CGAGACATTC GGCATCAGAC CCCAGTTCAG CTGGCACGTG GACCCTTTTG GAGCCTCTGC
     3481 CACCACACCT ACACTGTTTG CCCTGGCCGG CTTTAATGCC CACCTGGGCA GCAGAATCGA
     3541 CTACGATCTG AAGGCCGCCA TGCAGGAAGC TAGAGGCCTG CAGTTTGTGT GGCGGGGATC
     3601 TCCTAGCCTG AGCGAGAGAC AGGAAATCTT TACCCACATC ATGGACCAGT ACAGCTACTG
     3661 TACCCCCTCC CACATTCCCT TCAGCAACAG ATCCGGCTTC TACTGGAATG GGGTGGCCGT
     3721 GTTTCCCAAG CCTCCTCAGG ATGGGGTGTA CCCCAATATG TCTGAGCCCG TGACCCCCGC
     3781 CAACATCAAT CTGTATGCAG AGGCCCTGGT CGCCAATGTG AAACAGAGAG CCGCCTGGTT
     3841 CAGAACCCCT CATGTGCTGT GGCCTTGGGG CTGCGACAAG CAGTTTTTCA ACGCCAGCGT
     3901 GCAGTTCGCC AATATGGACC CCCTGCTGGA CCACATCAAT TCTCATGCTG CCGAGCTGGG
     3961 CGTGTCCGTG CAGTATGCTA CACTGGGCGA TTACTTCAGA GCCCTGCACG CCCTGAATGT
     4021 GACTTGGAGA GTGCGCGACC ACCACGATTT TCTGCCTTAC AGCACCGAGC CCTTTCAGGC
     4081 CTGGACCGGA TTCTACACCA GCAGATCTTC TCTGAAGGGC CTGGCTAGAA GGGCCAGCGC
     4141 ACTGCTGTAT GCCGGCGAGA GCATGTTCAC CAGATATCTC TGGCCTGCCC CAAGAGGCCA
     4201 CCTGGATCCT ACATGGGCTC TGCAGCAGCT CCAGCAGCTG CGCTGGGCTG TCAGTGAAGT
     4261 GCAGCACCAT GATGCCATCA CAGGCACCGA AAGCCCCAAA GTGCGGGATA TGTATGCCAC
     4321 CCATCTGGCC TCTGGCATGC TGGGAATGAG AAAGCTGATG GCCAGCATCG TGCTGGACGA
     4381 ACTCCAGCCT CAGGCTCCTA TGGCCGCTAG TTCTGATGCA GGACCAGCCG GACACTTCGC
     4441 CTCCGTGTAT AATCCACTGG CTTGGACCGT GACCACCATC GTGACCCTGA CAGTGGGATT
     4501 TCCAGGCGTC AGAGTGACAG ACGAAGCAGG CCATCCTGTG CCCAGCCAGA TCCAGAATAG
     4561 CACAGAGACA CCTAGCGCCT ACGACCTGCT GATTCTGACC ACAATCCCCG GCCTGAGCTA
     4621 CCGGCACTAC AACATTAGAC CTACCGCTGG CGCTCAGGAA GGCACACAGG AACCTGCTGC
     4681 TACAGTCGCC AGCACCCTCC AGTTTGGAAG AAGGCTGAGA AGAAGAACCA GCCACGCCGG
     4741 CAGATACCTG GTGCCCGTGG CCAATGATTG CTATATCGTG CTCCTGGATC AGGACACCAA
     4801 CCTGATGCAC AGCATCTGGG AGAGGCAGAG CAATAGAACC GTCCGCGTGA CCCAGGAATT
     4861 TCTGGAATAC CACGTGAACG GCGACGTGAA GCAGGGCCCC ATCAGCGACA ATTACCTGTT
     4921 TACACCTGGC AAAGCCGCCG TGCCTGCCTG GGAAGCTGTG GAAATGGAAA TTGTGGCCGG
     4981 ACAGCTGGTC ACCGAGATCA GACAGTACTT CTACCGGAAC ATGACCGCCC AGAACTACAC
     5041 CTACGCCATC AGGTCTAGAC TGACACACGT GCCCCAGGGA CATGATGGGG AACTGCTGTG
     5101 CCACCGGATC GAGCAGGAGT ATCAGGCAGG ACCTCTGGAA CTGAACCGGG AAGCCGTGCT
     5161 GAGAACCTCC ACCAATCTGA ACTCCCAGCA GGTGATCTAC TCCGACAACA ACGGCTACCA
     5221 GATGCAGAGA AGGCCCTACG TGTCCTACGT GAACAACAGC ATTGCCCGGA ACTACTACCC
     5281 TATGGTGCAG AGCGCCTTCA TGGAAGATGG CAAGAGCAGA CTCGTGCTGC TGAGCGAAAG
     5341 AGCCCACGGA ATCAGCTCTC AGGGCAATGG CCAGGTGGAA GTGATGCTGC ATCGGAGACT
     5401 GTGGAACAAC TTCGACTGGG ACCTGGGCTA CAACCTGACA CTGAACGATA CCTCCGTGGT
     5461 GCACCCTGTC CTGTGGCTCC TCCTGGGATC TTGGAGTCTG ACAACCGCTC TGAGACAGAG
     5521 ATCTGCACTG GCCCTGCAGC ATAGACCTGT GGTGCTGTTT GGAGATCTGG CCGGAACTGC
     5581 CCCTAAACTG CCAGGACCCC AGCAGCAGGA GGCTGTCACA CTGCCTCCAA ATCTGCACCT
     5641 CCAGATTCTG AGCATCCCTG GCTGGCGGTA CAGCAGCAAT CACACCGAAC ACAGCCAGAA
     5701 CCTGAGAAAG GGACATAGAG GCGAGGCCCA GGCTGACCTC AGAAGAGTCC TGCTGAGACT
     5761 GTACCACCTG TACGAAGTGG GCGAGGACCC TGTGCTCTCT CAGCCAGTGA CAGTGAATCT
     5821 CGAAGCCGTC CTGCAGGCTC TGGGTTCAGT GGTGGCTGTC GAGGAAAGAA GCCTGACAGG
     5881 CACTTGGGAT CTGTCCATGC TGCACCGGTG GTCTTGGAGA ACAGGACCTG GCAGACACAG
     5941 GGGCGATACC ACATCTCCTT CAAGACCTCC CGGCGGACCA ATCATCACCG TGCACCCCAA
     6001 AGAGATCCGG ACCTTCTTCA TCCACTTTCA GCAGCAGgaa aacttgtact ttcaaggctC
     6061 CAGCTTTCTT GTACAAAGTG GTATGAGCAA GGGCGAGGAA CTGTTCACCG GCGTGGTGCC
     6121 CATCCTGGTG GAACTGGATG GCGACGTGAA CGGCCACAAG TTCTCTGTGC GGGGAGAGGG
     6181 CGAAGGCGAC GCCACAAATG GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT
     6241 GCCCGTGCCT TGGCCTACCC TCGTGACCAC ACTGACCTAC GGCGTGCAGT GCTTCAGCAG
     6301 ATACCCCGAC CATATGAAGC GGCACGACTT CTTCAAGAGC GCCATGCCCG AGGGCTACGT
     6361 GCAGGAACGG ACCATCAGCT TCAAGGACGA CGGCACCTAC AAGACCAGAG CCGAAGTGAA
     6421 GTTCGAGGGC GACACCCTCG TGAACCGGAT CGAGCTGAAG GGCATCGATT TCAAAGAGGA
     6481 CGGCAACATC CTGGGCCACA AGCTGGAGTA CAACTTCAAC AGCCACAACG TGTACATCAC
     6541 CGCCGACAAG CAGAAGAACG GCATCAAGGC CAACTTCAAG ATCCGGCACA ACGTGGAAGA
     6601 TGGCAGCGTG CAGCTGGCCG ACCACTACCA GCAGAACACC CCCATCGGAG ATGGCCCCGT
     6661 GCTGCTGCCC GACAACCACT ACCTGAGCAC CCAGAGCGTG CTGAGCAAGG ACCCCAACGA
     6721 GAAGCGGGAC CACATGGTGC TGCTGGAATT CGTGACCGCC GCTGGCATCA CCCACGGCAT
     6781 GAGCGGCCTG AACGACATCT TCGAGGCCCA GAAAATCGAG TGGCACGAGC ACCACCATCA
     6841 TCACCATCAC CACTGATGAT TCGAAAAATA ATAAGGATCC AATCAACCTC TGGATTACAA
     6901 AATTTGTGAA AGATTGACTG GTATTCTTAA CTATGTTGCT CCTTTTACGC TATGTGGATA
     6961 CGCTGCTTTA ATGCCTTTGT ATCATGCTAT TGCTTCCCGT ATGGCTTTCA TTTTCTCCTC
     7021 CTTGTATAAA TCCTGGTTGC TGTCTCTTTA TGAGGAGTTG TGGCCCGTTG TCAGGCAACG
     7081 TGGCGTGGTG TGCACTGTGT TTGCTGACGC AACCCCCACT GGTTGGGGCA TTGCCACCAC
     7141 CTGTCAGCTC CTTTCCGGGA CTTTCGCTTT CCCCCTCCCT ATTGCCACGG CGGAACTCAT
     7201 CGCCGCCTGC CTTGCCCGCT GCTGGACAGG GGCTCGGCTG TTGGGCACTG ACAATTCCGT
     7261 GGTGTTGTCG GGGAAGCTGA CGTCCTTTCC ATGGCTGCTC GCCTGTGTTG CCACCTGGAT
     7321 TCTGCGCGGG ACGTCCTTCT GCTACGTCCC TTCGGCCCTC AATCCAGCGG ACCTTCCTTC
     7381 CCGCGGCCTG CTGCCGGCTC TGCGGCCTCT TCCGCGTCTT CGAGATCTGC CTCGACTGTG
     7441 CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG TGCCTTCCTT GACCCTGGAA
     7501 GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA TTGCATCGCA TTGTCTGAGT
     7561 AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA GCAAGGGGGA GGATTGGGAA
     7621 GACAATAGCA GGCATGCTGG GGACTCGAGT TAAGGGCGAA TTGCCGATTA GGATCTTCCT
     7681 AGAGCATGGC TACGTAGATA AGTAGCATGG CGGGTTAATC ATTAACTACA AGGAACCCCT
     7741 AGTGATGGAG TTGGCCACTC CCTCTCTGCG CGCTCGCTCG CTCACTGAGG CCGGGCGACC
     7801 AAAGGTCGCC CGACGCCCGG GCTTTGCCCG GGCGGCCTCA GTGAGCGAGC GAGCGCGCAG
     7861 CCTTAATTAA GAGCTCATGG CGCGCCTAGG CCTTGACGGC CTTCCGCCAA TTCGCCCTAT
     7921 AGTGAGTCGT ATTACGTCGC GCTCACTGGC CGTCGTTTTA CAACGTCGTG ACTGGGAAAA
     7981 CCCTGGCGTT ACCCAACTTA ATCGCCTTGC AGCACATCCC CCTTTCGCCA GCTGGCGTAA
     8041 TAGCGAAGAG GCCCGCACCG AAACGCCCTT CCCAACAGTT GCGCAGCCTG AATGGCGAAT
     8101 GGGAGCGCCC TGTAGCGGCC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG
     8161 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG
     8221 AATTTTAACA AAATATTAAC GCTTACAATT TAG
//

